• Home »
  • Next generation seq.
  • Terms
  • Applications
  • Schemes »
    • Fragment libr. prep
    • Mate-Paired libr. prep
    • Clonal amplification
    • Polymerization-based sequencing
    • Ligation-based sequencing
• Illumina »
  • Library »
    • Fragment
    • Mate-Paired
    • Mate-Paired Long
    • Library amplification
  • Cluster Station »
    • Cluster generation
    • Cluster control
    • FlowCell processing
    • Multiple primer hyb.
    • DECON wash
  • 2G analyzer »
    • Focusing
    • Reagents
    • Sequencing (first read)
    • Sequencing (PE read)
  • Data analysis
  • Illumina materials
• SOLiD »
  • Library »
    • Fragment
    • Mate-Paired
    • Mate-Paired Long
    • Library amplification
  • ePCR »
    • ePCR amplification
    • Bead wash
    • Bead enrichment
    • 3'-end modification
  • Sequencing »
    • Bead deposition
    • Flowcell installation
    • QC-run
    • Sequencing run
    • Enzyme and Primer plates
  • SOLiD Kits handling
  • SOLiD materials
• General »
  • Solutions
  • Consumables
  • Kits and enzymes
  • DNA »
    • RNase treatment
    • Shearing
    • Purification
  • RNA »
    • mRNA purification on Dynabeads
    • ds cDNA synthesis
  • Oligonucleotides
  • NA quantitation
  • NA electrophoresis
  • Agarose
  • PAAG
  • Data analysis »
    • UNIX comands

Consumables

Laboratory tubes and tips Prices Laboratory plastic  Comments

Laboratory tubes and tips

Different tubes and tips

• There are two advantages of autoclaved tips & tubes: (i) they are sterile, (ii) we not need to pay for them. In all other respects they are worse. In most of our applications we not need sterility. Tubes were dispensed in boxes of questionable purity. They were heated up to significant temperature. Autoclave steam condensed on them and then was dried out in dry thermostate.
• Autoclaved tubes and tips should be used for: molecular cloning and microbiology (because they are sterile) and for handling of not important non-amplifiable material: routine titrations, handling of markers, preparation of samples for gel-loading, plasmid purifications, phenol-chlorophorm extractions; concentration measurements (spectrophotometer, nanodrop); etc.
• Tips with filters should be used for work with "PCR-contamination sensitive" and "important" material: preparation of aliquotes of enzymes, oligos, buffers; setting up of PCR reactions; handling of amplified material, etc.
• LoBind tubes are for surface-sensitive applications: (i) all operations with paramagnetic and polystyrene particles; (ii) storage of very diluted solutions: libraries, oligos, proteins; (iii) storage of expensive or sensitive enzymes.
• Do not use coloured tubes. Pellet is less visible. They also may inhibit some chemical reactions (Maxam-Gilbert sequencing)
• Soft polyethylene tubes are not suitable for centrifugatin!





Tubes

volume € per tube type notes 0.2ml tubes   non-autoclaved thin walls to accelerate heat transfer; caps not good for numerous opening/closing, so do not use these tubes for storage; not good for water bath, high-speed centrifugation, rotation, etc. 0.2ml strips 74¢ per strip non-autoclaved autoclaved 0.5ml 3.9¢ transparent larger tubes are too large for 2µl tips, the best choice for storage of enzymes, oligos, libraries, etc. 7.6¢ LoBind ~0.13¢ non-autoclaved autoclaved 1.7ml 4.1¢ LoBind general use tubes ~0.32¢ non-autoclaved autoclaved 2ml ~0.43¢ non-autoclaved large-volume modification of 1.7ml tubes; if compare with conical tubes: mixing is better, pellet is less stable autoclaved 2ml w/o caps 2.4¢ with volume marks collection tubes for spin-columns 1.6¢ w/o volume marks 5ml 12¢ sterile intermediate volume between 2ml and 50ml 8¢ non-sterile 14ml 7.9¢ sterile very inconvenient tubes, it is difficult to take last portions without touching internal surface by pipette 20ml 9.5¢ sterile   30ml 2.6¢ non-sterile   50ml 7.9¢ sterile general use tubes 100ml 12¢ non-sterile








Prices for common consumables and procedures

item price per one [€] 2% HR agarose gel, full* 11 2% HR agarose gel, 2/3* 8.7 ULTRA TURRAX tube ~5.5 96-well plate 5.3 SYBR Green I/II staining sol., 100ml 5 48-well plate 3.2 Stericup 0.25-1L 3-6 SYBR GOLD staining sol., 100ml 2.7 0.45-5µm 0.7ml spin filter 2.6 Steriflip, 50ml 2.2 MinElute cleaning ~1.7 QIAquick cleaning ~1.6 gel with Gel Red/Green, full* ~1.5 0.7% agarose gel, full* 1.1 Combitip, sterile 1





item price per one [¢] 12% acrylamide gel (40% AA:BIS stock) ~100 Sealing foil for 1 plate 50-60 Combitip, non-sterile 56 12% acrylamide gel (solid AA:BIS) ~50 1.5% agarose gel, full* ~50 Qubit measurement 38 Positive-displacement tip 28 Scalpel 27 Aluminiumfolie (25cm) ~13 Gloves (pair) ~15 Syringe, 1-10ml 10-15 20G needles ~8 pH strip 6-12 Polyethylene bag 5-15 G-50 for one column ~10 Tip with filter ~5 Laser Tough-Spot 2

^* agarose gel: 140ml




Laboratory plastic

plastic properties products Polypropylene (PP) ~0.90g/ml; autoclavable; high mechanical strength, low binding capacity to proteins and nucleic acids tips, tubes, plates, syringes, bottles Polystyrene (PS) 1.04g/ml; not autoclavable; transparent, high protein binding capacity Petri dishes, filter units, tissue culture flasks, serological pipets, microplates, immunological plates, beads, CD, DVD Polycarbonate (PC) 1.20-1.22g/ml; can be weakened by repeated autoclaving; transparent, temperature stable PCR plates, ultracentrifuge tubes, CD, DVD High density polyethylene (HDPE) ~0.95g/ml; can be autoclaved at 120°C for 20 min; higher chemical and thermal resistance if compare with polystyrene bottles, caps Low density polyethylene (LDPE) ~0.92g/ml; not autoclavable; higher chemical and thermal resistance if compare with polystyren bottles, caps Polytetrafluoroethylene (PTFE) ~1.70g/ml; autoclavable; high chemical and thermal resistance spacers, combs, filters Acrylic glass (plexiglas, acrylite, etc.) ~1.19g/ml; not autoclavable, transparent electrophoresis chambers, agarose plates UV-Star resin   UV-transparent plastic cuvettes

Other types of plastic are used for ultracentrifuge tubes, filters, meshes.



Chemical&thermal resistance of different types of plastic

factor PP PS HDPE LDPE UV-Star Acrylic glass PTFE temperature [°C] -200 ÷ +120 -20 ÷ +60 -50 ÷ +120 -50 ÷ +60 -80 ÷ +40 -80 ÷ +80 -100 ÷ +250 compatible with all plastics Ammonia acetate, DMSO, Formamide, Glucose, Glycerol, NaCl, NaOH, Urea Acetic acid, 90% 20°C 20°C 50°C 20°C 20°C 20°C 50°C Acetone 20°C 20°C 50°C 20°C 20°C 20°C 50°C Ammonia, 25 % 50°C 20°C 50°C 50°C 20°C 20°C 50°C Chloroform, 100 % 20°C 20°C 20°C 20°C   20°C 50°C Diethyl ether 20°C 20°C 20°C 20°C   20°C 50°C Ethanol, 96 % 50°C 50°C 20°C 20°C 20°C 20°C 50°C Formaldehyde, 40 % 20°C 20°C 20°C 20°C     50°C Heptane 20°C 20°C 20°C 20°C 20°C 20°C   Hydrochloric acid, conc. 50°C 20°C 50°C 50°C 20°C 20°C 50°C Isobutanol 50°C 20°C 50°C 50°C       Isopropanol 50°C 20°C 50°C 50°C 20°C     Liquid paraffin 20°C 50°C 50°C 20°C   20°C   Methanol 50°C 20°C 50°C 50°C 20°C 20°C 50°C Phenol, 100 % 50°C 20°C 20°C 20°C   20°C 50°C Phosphoric acid, 85 % 20°C 50°C 50°C 50°C     50°C Sodium acetate 50°C 20°C 50°C 50°C   20°C 50°C Sodium hypochloride 20°C 50°C 20°C 20°C 20°C 20°C 50°C Sulphuric acid, 60 % 20°C 20°C 20°C 20°C 20°C   50°C Sulphuric acid, conc. 20°C 20°C 20°C 20°C   20°C 50°C Toluene 20°C 20°C 20°C 20°C   20°C 50°C Trichloroacetic acid 50°C 20°C 20°C 20°C   20°C 50°C Xylene 20°C 20°C 20°C 20°C   20°C 50°C


see also:
• Material Chemical Compatibility
• Chemical resistance charts
• Chemical and thermal resistance of Polypropylene, Polystyrene, LDPE, HDPE, EVA and UV-Star
• Acrylite, chemical resistance in general use